Plasma Treated Effect of Poly (Lactic-co-Glycolic acid) (PLGA) Scaffold on Proliferation and Differentiation of Preadipocyte

J Korean Wound Management Soc > Volume 1(1); 2005 > Article

Original

Journal of the Korean Wound Management Society 2005;1(1): 38-45.

PLGA 지지체 표면에 대한 Plasma처리가 지방전구세포의 분화에 미치는 영향

이종원·홍정근·오득영·이백권·안상태·한동근¹·한기택

가톨릭대학교 의과대학 성형외과학교실, ¹한국과학기술연구원 생체재료연구센터

Plasma Treated Effect of Poly (Lactic-co-Glycolic acid) (PLGA) Scaffold on Proliferation and Differentiation of Preadipocyte

Jong Won Rhie, Jeong Geun Hong, Deuk Young Oh, Paik Kwon Lee, Sang Tae Ahn, Dong Keun Han,¹ Ki Taik Han

Department of Plastic Surgery, College of Medicine, The Catholic University of Korea, ¹Biomaterials Research Center, Korea Institute of Science and Technology, Seoul, Korea

Published online: 30 November 2005.

ABSTRACT

Human preadipocytes attachment to poly (lactide-co-glycolide) [PLGA] sponge was examined in vitro. This study was performed to investigate the effect of surface modification of poly (lactic-co-glycolic acid) (PLGA) scaffold by plasma glow discharge treatment on the adhesion, proliferation and adipogenesis of human preadipocytes, and not plasma glow discharge treatment. Preadipocytes were isolated from human subcutaneous adipose tissue obtained by lipectomy and liposuction. The amounts of DNA for cell proliferation rate of three-dimensional cultured group (PLGA) were higher than monolayer cultured group. At the beginning of culture, the amounts of DNA of preadipocyte in PLGA with plasma treated were higher than those without plasma treated. There was no significant difference of DNA amount between PLGA with plasma treatment and those without plasma treatment after 7 day culture. Messenger RNA of PPAR- gamma and LPL from all the preadipocyte cultured by differential medium were expressed by RT-PCR. On the 7 th and 11th day after culture, m-RNA expression of PLGA with plasma treatment were stronger than those of PLGA without plasma treatment. These results suggest that plasma treatment of PLGA sponge not only could increase the adhesion property and proliferation of preadipocytes, but also provide suitable environment for differentiation of preadipocytes. (J Korean Wound Care Soc 2005;1:38-45)

Key Words: Cells cultured, Surface property, Plasma glow discharge, Polymer, Preadipocytes