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Journal of the Korean Wound Management Society 2012;8(1): 16-22. |
| Effects of Prostaglandin E1 on Proliferation and Collagen Synthesis in Stromal Vascular Fraction Cells of Human Adipose Tissue |
| Na Hyun Hwang, Huanna Yang, Seung-Kyu Han, Woo-Kyung Kim |
| Department of Plastic Surgery, Korea University College of Medicine, Seoul, Korea |
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Published online: 30 May 2012. |
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| ABSTRACT |
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Prostaglandin E1 (PGE1) has been therapeutically appraised with respect to its efficacy in the treatment of various diseases due to its effects on vasodilatation and anti-platelet aggregation. Although there have been a number of studies that focused on the role and mechanism in different tissues and cells, there are very few reports on its effects on stromal vascular fraction (SVF) cells of adipose tissues, which are currently focused on in regenerative medicine. The purpose of this study was to investigate the role of PGE1 in the activity of SVF cells. SVF cells were obtained from liposuctioned human adipose tissue. The SVF cells were treated with five concentrations of PGE1. The concentration of SVF cells were 0 ՌM, 3 ՌM, 6 ՌM, 9 ՌM, and 12 ՌM, respectively. Following PGE1 treatment, cell proliferation was measured by CCK-8 assay, and collagen synthesis by CICP method. Subsequently, the result of each group was compared. SVF cell proliferation significantly increased after being treated with PGE1 (P＜0.05 in the Kruskal-Wallis Test). In contrast, collagen type 1 synthesis decreased (P＜0.05 in the Kruskal-Wallis Test). These changes were dose-dependent. PGE1 is a dose-dependent reagent which stimulates SVF cell proliferation, but suppresses collagen synthesis. (J Korean Wound Management Soc 2012;8:16-22) |
| Key Words:
Prostaglandin E1, Adipose tissue, Collagen |
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