Co-supplementation Effect of GM-CSF and Vitamin-C on Wound Healing In Vitro

J Korean Wound Management Soc > Volume 2(1); 2006 > Article

Original

Journal of the Korean Wound Management Society 2006;2(1): 28-33.

창상치유를 위한 비타민-C와 GM-CSF의 혼합효과

홍용택ㆍ한승규ㆍ김정배¹·김우경

고려대학교 의과대학 성형외과학교실, ¹건양대학교 의과대학 성형외과학교실

Co-supplementation Effect of GM-CSF and Vitamin-C on Wound Healing In Vitro

Yong-Taek Hong, Seung-Kyu Han, Jeong-Bae Kim¹, Woo-Kyung Kim

Department of Plastic Surgery, Korea University College of Medicine, Seoul, ¹Department of Plastic Surgery, Konyang University College of Medicine, Daejeon, Korea

Published online: 31 May 2005.

ABSTRACT

Granulocyte-macrophage colony-stimulating factor (GM-CSF) is naturally occuring protein that has the healing effect on normal wound as well as infected nonhealing wounds and ulcers. Vitamin-C (ascorbic acid) has also been shown to stimulate fibroblast stimulation and collagen synthesis. However, there are no reports about the effect of GM-CSF and ascorbic acid co-supplementation on wound healing. Therefore, in this report, we examined the effect of GM-CSF and ascorbic acid co-supplementation on the proliferation of human dermal fibroblasts and collagen synthesis which play a crucial role in wound healing process in vitro. To determine an optimal GM-CSF and ascorbic acid concentration for human dermal fibroblast proliferation and collagen synthesis, the cells were incubated with varying concentrations of GM-CSF and ascorbic acid. The activity of fibroblast proliferation was determined by MTT assay. To measure the amount of collagen production, the collagen type I carboxy-terminal propeptide enzyme immunoassay was used. The best fibroblast proliferation was seen at the co-supplementation of 0.5μg/ml GM-CSF with 25μg/ml ascorbic acid and 1μg/ml GM-CSF with 12.5μg/ml ascorbic acid. Maximal stimulation of collagen synthesis was seen at the co- supplementation of 1μg/ml GM-CSF with 12.5μg/ml ascorbic acid. The collagen synthesis per cell was also maximal at concentration of 1μg/ml GM-CSF with 12.5μg/ml ascorbic acid. This results demonstrated that GM-CSF and ascorbic acid co-supplementation increased human dermal fibroblast proliferation and collagen synthesis and the concentration was critically important factor in vitro. (J Korean Wound Care Soc 2006;2:28-33)

Key Words: GM-CSF, Vitamin-C